Genome. 2010 Jul;53(7):568-74.

Whole human exome capture for high-throughput sequencing.

Kim DW, Nam SH, Kim RN, Choi SH, Park HS.

Source

Division of Malaria and Parasitic Diseases, National Institute of Health, Seoul, Korea.

Abstract

We captured the whole human exome by hybridization using synthesized oligonucleotides, based on a high-density microarray design, and we sequenced those captured human exons using high-throughput sequencing on a Genome Sequencer FLX instrument. Of the uniquely mapped reads, 71% fell within target regions, and these corresponded to coverage of 94% of human genes, 87% of exons, and 70% of the total base-pair length of the CCDS set. Our study provides strong evidence for the practical usefulness of this method on a genome-wide scale, showing the resequenced whole human exome database with 501 microRNAs and 307 novel SNPs.

PMID:: 20616878; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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MicroRNAs

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