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J Neuroimmunol. 2010 Oct 8;227(1-2):26-34. doi: 10.1016/j.jneuroim.2010.06.007. Epub 2010 Jul 7.

Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1β: evidence for opioid-immune crosstalk.

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  • 1Department of Pharmacology and Physiology, Oklahoma State University-Center for Health Sciences, 1111 West 17(th) Street, Tulsa, OK 74107, USA.


Treatment of SK-N-SH cells with morphine and interleukin-1beta (IL-1β) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1β increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1β-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1β and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.

Copyright © 2010 Elsevier B.V. All rights reserved.

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