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    J Biomed Opt. 2010 May-Jun;15(3):030505. doi: 10.1117/1.3420209.

    Multiphoton microscopy with near infrared contrast agents.

    Yazdanfar S, Joo C, Zhan C, Berezin MY, Akers WJ, Achilefu S.

    Abstract

    While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

    PMID:
    20614991
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC2881927
    Free PMC Article

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