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J Biomed Opt. 2010 May-Jun;15(3):030505. doi: 10.1117/1.3420209.

Multiphoton microscopy with near infrared contrast agents.

Abstract

While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

PMID:
20614991
[PubMed - indexed for MEDLINE]
PMCID:
PMC2881927
Free PMC Article

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