Structure and function of P19, a high-affinity iron transporter of the human pathogen Campylobacter jejuni

J Mol Biol. 2010 Aug 27;401(4):590-604. doi: 10.1016/j.jmb.2010.06.038. Epub 2010 Jun 30.

Abstract

Campylobacter jejuni, a major cause of acute bacterial diarrhea in humans, expresses numerous proteins to import diverse forms of essential iron. The expression of p19 and an adjacent iron transporter homologue (ftr1) is strongly induced upon iron limitation, suggesting a function in iron acquisition. Here, we show that the loss of P19 alone is detrimental to growth on iron-restricted media. Furthermore, metal binding analysis demonstrates that recombinant P19 has distinct copper and iron binding sites. Crystal structures of P19 have been solved to 1.41 A resolution, revealing an immunoglobulin-like fold. A P19 homodimer in which both monomers contribute ligands to two equivalent copper sites located adjacent to methionine-rich patches is observed. Copper coordination occurs via three histidine residues (His42, His95, and His132) and Met88. A solvent channel lined with conserved acidic residues leads to the copper site. Soaking crystals with a solution of manganese as iron analog reveals a second metal binding site in this solvent channel (metal-metal distance, 7.7 A). Glu44 lies between the metal sites and displays multiple conformations in the crystal structures, suggesting a role in regulating metal-metal interaction. Dimerization is shown to be metal dependent in vitro and is detected in vivo by cross-linking.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism
  • Binding Sites
  • Campylobacter jejuni / metabolism*
  • Campylobacter jejuni / pathogenicity
  • Copper / chemistry
  • Copper / metabolism
  • Crystallography, X-Ray
  • Humans
  • Iron / metabolism*
  • Ligands
  • Manganese / metabolism
  • Membrane Transport Proteins / chemistry*
  • Membrane Transport Proteins / metabolism
  • Models, Molecular
  • Molecular Sequence Data
  • Structure-Activity Relationship

Substances

  • Bacterial Proteins
  • Ligands
  • Membrane Transport Proteins
  • Manganese
  • Copper
  • Iron

Associated data

  • PDB/3LZL
  • PDB/3LZN
  • PDB/3LZO
  • PDB/3LZP
  • PDB/3LZQ
  • PDB/3LZR