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J Gastroenterol Hepatol. 2010 May;25 Suppl 1:S90-4. doi: 10.1111/j.1440-1746.2009.06213.x.

Assessment of in vitro biofilm formation by Helicobacter pylori.

Author information

  • 1Department of Infectious Disease, Kyorin University School of Medicine, Shinkawa, Tokyo, Japan. yonezawa@ks.kyorin-u.ac.jp

Abstract

BACKGROUND AND AIMS:

Biofilms are surface-bound communities of bacterial cells that are implicated in their survival. As with various bacteria studied to date, Helicobacter pylori can have an alternate lifestyle as a biofilm. We previously reported that strain TK1402 showed a strong biofilm-forming ability in vitro. However, the mechanisms of its biofilm development remain unclear. We analyzed the basic characteristics of the biofilm-forming ability in strain TK1402.

METHODS:

In order to characterize the biofilm-forming ability of the H. pylori strains, auto-aggregation, motility and hydrophobicity, which are important factors in biofilm formation by other bacteria, were analyzed. Further, we tested whether cell growth participated in biofilm formation in strain TK1402.

RESULTS:

There were no significant differences in the auto-aggregation, motility and hydrophobicity of strain TK1402 compared with the other strains. On the other hand, pre-culture of this strain for 24-48 h resulted in decreased biofilm formation.

CONCLUSION:

TK1402 is a strong biofilm-forming strain of H. pylori in Brucella broth supplemented with 7% fetal calf serum. It is possible that biofilm-forming cell growth is a principal factor in biofilm development.

PMID:
20586874
[PubMed - indexed for MEDLINE]
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