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BMC Biol. 2010 Jun 28;8:91. doi: 10.1186/1741-7007-8-91.

Disruption of insulin signalling preserves bioenergetic competence of mitochondria in ageing Caenorhabditis elegans.

Author information

  • 1Department of Biology, Ghent University, K L Ledeganckstraat 35, Ghent B-9000, Belgium. Bart.Braeckman@UGent.be.

Abstract

BACKGROUND:

The gene daf-2 encodes the single insulin/insulin growth factor-1-like receptor of Caenorhabditis elegans. The reduction-of-function allele e1370 induces several metabolic alterations and doubles lifespan.

RESULTS:

We found that the e1370 mutation alters aerobic energy production substantially. In wild-type worms the abundance of key mitochondrial proteins declines with age, accompanied by a dramatic decrease in energy production, although the mitochondrial mass, inferred from the mitochondrial DNA copy number, remains unaltered. In contrast, the age-dependent decrease of both key mitochondrial proteins and bioenergetic competence is considerably attenuated in daf-2(e1370) adult animals. The increase in daf-2(e1370) mitochondrial competence is associated with a higher membrane potential and increased reactive oxygen species production, but with little damage to mitochondrial protein or DNA. Together these results point to a higher energetic efficiency of daf-2(e1370) animals.

CONCLUSIONS:

We conclude that low daf-2 function alters the overall rate of ageing by a yet unidentified mechanism with an indirect protective effect on mitochondrial function.

PMID:
20584279
[PubMed - indexed for MEDLINE]
PMCID:
PMC2914644
Free PMC Article

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