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Cell Mol Life Sci. 2010 Oct;67(19):3355-69. doi: 10.1007/s00018-010-0434-3. Epub 2010 Jun 27.

SAM domain-dependent activity of PfTKL3, an essential tyrosine kinase-like kinase of the human malaria parasite Plasmodium falciparum.

Author information

  • 1Inserm U609, Wellcome Trust Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, 120 University Place, Glasgow, Scotland G12 8TA, UK.

Abstract

Over the last decade, several protein kinases inhibitors have reached the market for cancer chemotherapy. The kinomes of pathogens represent potentially attractive targets in infectious diseases. The functions of the majority of protein kinases of Plasmodium falciparum, the parasitic protist responsible for the most virulent form of human malaria, remain unknown. Here we present a thorough characterisation of PfTKL3 (PF13_0258), an enzyme that belongs to the tyrosine kinase-like kinase (TKL) group. We demonstrate by reverse genetics that PfTKL3 is essential for asexual parasite proliferation in human erythrocytes. PfTKL3 is expressed in both asexual and gametocytes stages, and in the latter the protein co-localises with cytoskeleton microtubules. Recombinant PfTKL3 displays in vitro autophosphorylation activity and is able to phosphorylate exogenous substrates, and both activities are dramatically dependent on the presence of an N-terminal "sterile alpha-motif" domain. This study identifies PfTKL3 as a validated drug target amenable to high-throughput screening.

PMID:
20582613
[PubMed - indexed for MEDLINE]
PMCID:
PMC2933843
Free PMC Article

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