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Nat Methods. 2010 Jul;7(7):516-8. doi: 10.1038/nmeth.1471. Epub 2010 Jun 27.

Simultaneous intracellular chloride and pH measurements using a GFP-based sensor.

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  • 1National Enterprise for nanoScience and nanoTechnology, Istituto Nanoscienze, Consiglio Nazionale delle Ricerche and Scuola Normale Superiore, Pisa, Italy. daniele.arosio@cnr.it

Abstract

Chloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting ClopHensor to these intracellular compartments.

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