Ther Drug Monit. 1991 Jan;13(1):69-72.

The assay of clozapine and N-desmethylclozapine in human plasma by high-performance liquid chromatography.

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Department of Psychiatry, College of Medicine, University of Iowa, Iowa City 52242.

Abstract

A high performance liquid chromatographic method for the simultaneous quantitation of clozapine--a member of the dibenzodiazepine class of antipsychotic drugs--and its reduced metabolite in human plasma has been developed. Clozapine and N-desmethylclozapine are concentrated from blood samples by liquid-liquid extraction, followed by reverse-phase liquid chromatography. Accuracy of the determination is ensured by application of an internal standard, protriptyline, which is closely related to clozapine. A detection limit of 15 ng/mL for clozapine and 30 ng/ml for N-desmethylclozapine was achieved.

PMID:: 2057995; [PubMed - indexed for MEDLINE]

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The assay of clozapine and N-desmethylclozapine in human plasma by high-performance liquid chromatography.

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