Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Phys Chem Chem Phys. 2010 Sep 14;12(34):10018-26. doi: 10.1039/c0cp00305k. Epub 2010 Jun 24.

Monolayer anthracene and anthraquinone modified electrodes as platforms for Trametes hirsuta laccase immobilisation.

Author information

  • 1School of Chemistry, University of Southampton, Southampton, SO17 1BJ, UK.

Abstract

Surface modification techniques are essential to the construction of enzyme based elements of biofuel cells and biosensors. In this article we report on the preparation and characterisation of modified carbon electrodes which were used as supports for the immobilisation of laccase from Trametes hirsuta. The electrodes were electrochemically modified with diamine or diazonium linkers followed by attachment of either anthracene or anthraquinone head groups using solid phase chemical methodology. These well defined surfaces were found to effectively bind laccase and to provide direct electrical contact to the enzyme active site, as evidenced by XPS, EIS and voltammetry, respectively. The influence of the type of linker and head group on enzyme binding and bioelectrocatalytic activity are evaluated.

PMID:
20577679
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Royal Society of Chemistry
    Loading ...
    Write to the Help Desk