Abstract
We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent Ca(2+) indicator that can be covalently linked to SNAP-tag fusion proteins and retains its Ca(2+) sensing ability after coupling to protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Calcium / analysis*
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / metabolism*
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Fura-2 / chemical synthesis
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Fura-2 / chemistry*
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Fura-2 / metabolism*
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Guanine / chemistry
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Proteins / metabolism*
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Spectrometry, Fluorescence
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Staining and Labeling / methods*
Substances
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Fluorescent Dyes
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Proteins
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Guanine
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Calcium
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Fura-2