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Genome Biol. 2010;11(6):R61. doi: 10.1186/gb-2010-11-6-r61. Epub 2010 Jun 15.

Design and evaluation of genome-wide libraries for RNA interference screens.

Author information

  • 1German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics and University of Heidelberg, Department of Cell and Molecular Biology, Faculty of Medicine Mannheim, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany.

Abstract

RNA interference (RNAi) screens have enabled the systematic analysis of many biological processes in cultured cells and whole organisms. The success of such screens and the interpretation of the data depend on the stringent design of RNAi libraries. We describe and validate NEXT-RNAi, a software for the automated design and evaluation of RNAi sequences on a genome-wide scale. NEXT-RNAi is implemented as open-source software and is accessible at http://www.nextrnai.org/.

PMID:
20550664
[PubMed - indexed for MEDLINE]
PMCID:
PMC2911109
Free PMC Article

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