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J Infect Dis. 2010 Jul 15;202(2):184-91. doi: 10.1086/653823.

Human polyomavirus JC (JCV) infection of human B lymphocytes: a possible mechanism for JCV transmigration across the blood-brain barrier.

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  • 1Retrovirology Research Laboratory, Department of Tropical Medicine, Medical Microbiology, and Pharmacology, John A Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii 96813, USA.

Abstract

It has been suggested that JC virus (JCV) might travel to the central nervous system in infected B cells. Moreover, recent data suggest the presence of JCV in bone marrow plasma cells. However, the evidence for infection and replication of JCV in B cells is unclear. To address this question, we infected Epstein-Barr virus-transformed B cells with JCV and found that the viral genome decreased >1000-fold from days 0 to 20 after infection, which concurred with the absence of viral early and late messenger RNA transcripts and proteins. However, immunofluorescent images of B cells infected with fluorescein isothiocyanate-conjugated JCV demonstrated that JCV enters the B cells, and DNase protection assay confirmed the presence of intact JCV virions inside the B cells. Moreover, JCV-infected B cells were able to transmit infection to naive glial cells. These data confirm that JCV nonproductively infects B cells and possibly uses them as a vehicle for transmigration across the blood-brain barrier.

PMID:
20550458
[PubMed - indexed for MEDLINE]
PMCID:
PMC2928669
Free PMC Article

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