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Lett Appl Microbiol. 2010 Aug;51(2):123-30. doi: 10.1111/j.1472-765X.2010.02859.x. Epub 2010 Apr 29.

Cell-bound exopolysaccharide from probiotic bacteria induces autophagic cell death of tumour cells.

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  • 1Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

Abstract

AIM:

Lactic acid bacteria (LAB) are beneficial micro-organisms that have been associated with several probiotic effects in both humans and animals. Here, using proteome analysis, we investigate the antitumour effects of cell-bound exopolysaccharides (cb-EPS) isolated from Lactobacillus acidophilus 606 on colon cancer cells and explore the proteins critical for their antitumour activity.

METHODS AND RESULTS:

cb-EPS inhibited the proliferation of HT-29 colon cancer cells by directly affecting cell morphology and not the cell cycle. Using two-dimensional polyacrylamide gel electrophoresis coupled with matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and immunoblot analysis, we found that cb-EPS dramatically induced Beclin-1 and GRP78, and affected Bcl-2 and Bak regulation.

CONCLUSIONS:

The results of this study indicate that cb-EPS are antitumourigenic against HT-29 colon cancer cells and that this activity is because of the activation of autophagic cell death promoted directly by the induction of Beclin-1 and GRP78, as well as indirectly through the induction of Bcl-2 and Bak.

SIGNIFICANCE AND IMPACT OF THE STUDY:

These results may contribute to understanding the novel mechanisms by which probiotic bacteria induce tumour cell death via autophagy.

PMID:
20536712
[PubMed - indexed for MEDLINE]
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