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Nat Methods. 2010 Jul;7(7):554-9. doi: 10.1038/nmeth.1463. Epub 2010 May 30.

Enhanced neuronal RNAi in C. elegans using SID-1.

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  • 1Department of Biological Sciences, Columbia University, New York, New York, USA.

Abstract

We expressed SID-1, a transmembrane protein from Caenorhabditis elegans that is required for systemic RNA interference (RNAi), in C. elegans neurons. This expression increased the response of neurons to double-stranded (ds)RNA delivered by feeding. Mutations in the lin-15b and lin-35 genes enhanced this effect. Worms expressing neuronal SID-1 showed RNAi phenotypes when fed with bacteria expressing dsRNA for known neuronal genes and for uncharacterized genes with no previously known neuronal phenotypes. Neuronal expression of sid-1 decreased nonneuronal RNAi, suggesting that neurons expressing transgenic sid-1(+) served as a sink for dsRNA. This effect, or a sid-1(-) background, can be used to uncover neuronal defects for lethal genes. Expression of sid-1(+) from cell-specific promoters in sid-1 mutants results in cell-specific feeding RNAi. We used these strains to identify a role for integrin signaling genes in mechanosensation.

PMID:
20512143
[PubMed - indexed for MEDLINE]
PMCID:
PMC2894993
Free PMC Article

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