J Immunol. 1991 Jul 1;147(1):369-74.

Cloning of the murine lymphocyte function-associated molecule-1 alpha-subunit and its expression in COS cells.

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Center for Blood Research, Harvard Medical School, Boston, MA 02115.

Abstract

The lymphocyte function-associated molecule 1 (LFA-1, CD11a/CD18) is an integrin that mediates adhesion of immune cells by interaction with two members of the Ig superfamily, ICAM-1 and ICAM-2. LFA-1 consists of an alpha subunit (Mr = 180,000) and a beta subunit (Mr = 95,000). We report here the isolation and expression of the murine alpha subunit cDNA (GenBank accession no. M60778). The deduced sequence comprises a 1061 amino acid extracellular domain, a 29 amino acid transmembrane region, and a 50 amino acid cytoplasmic domain. It has a 72% amino acid identity with its human counterpart and 34% identity with the murine Mac-1 alpha subunit. The murine LFA-1 alpha subunit could be expressed on the cell surface of a fibroblastoid cell line, COS, by cotransfection with either the human or murine beta subunit cDNA.

PMID:: 2051027; [PubMed - indexed for MEDLINE]

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Primary structure of the leukocyte function-associated molecule-1 alpha subunit: an integrin with an embedded domain defining a protein superfamily. [J Cell Biol. 1989]
Primary structure of the leukocyte function-associated molecule-1 alpha subunit: an integrin with an embedded domain defining a protein superfamily.
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Cited by 12 PubMed Central articles

The wild boar (Sus scrofa) lymphocyte function-associated antigen-1 (CD11a/CD18) receptor: cDNA sequencing, structure analysis and comparison with homologues. [BMC Vet Res. 2007]
The wild boar (Sus scrofa) lymphocyte function-associated antigen-1 (CD11a/CD18) receptor: cDNA sequencing, structure analysis and comparison with homologues.
Bergh PG, Zecchinon LL, Fett T, Desmecht DJ. BMC Vet Res. 2007 Oct 15; 3:27. Epub 2007 Oct 15.
Aggregatibacter actinomycetemcomitans leukotoxin requires beta-sheets 1 and 2 of the human CD11a beta-propeller for cytotoxicity. [Cell Microbiol. 2007]
Aggregatibacter actinomycetemcomitans leukotoxin requires beta-sheets 1 and 2 of the human CD11a beta-propeller for cytotoxicity.
Kieba IR, Fong KP, Tang HY, Hoffman KE, Speicher DW, Klickstein LB, Lally ET. Cell Microbiol. 2007 Nov; 9(11):2689-99. Epub 2007 Jun 24.
The CD11a partner in Sus scrofa lymphocyte function-associated antigen-1 (LFA-1): mRNA cloning, structure analysis and comparison with mammalian homologues. [BMC Vet Res. 2005]
The CD11a partner in Sus scrofa lymphocyte function-associated antigen-1 (LFA-1): mRNA cloning, structure analysis and comparison with mammalian homologues.
Vanden Bergh PG, Fett T, Zecchinon LL, Thomas AV, Desmecht DJ. BMC Vet Res. 2005 Oct 10; 1:5. Epub 2005 Oct 10.

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Cloning of the murine lymphocyte function-associated molecule-1 alpha-subunit an...
Cloning of the murine lymphocyte function-associated molecule-1 alpha-subunit and its expression in COS cells.
J Immunol. 1991 Jul 1 ;147(1):369-74.

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