Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
Genome Res. 2010 Sep;20(9):1165-73. doi: 10.1101/gr.101360.109. Epub 2010 May 27.

Assembly of large genomes using second-generation sequencing.

Author information

  • 1Center for Bioinformatics and Computational Biology, University of Maryland, College Park, Maryland 20742, USA.


Second-generation sequencing technology can now be used to sequence an entire human genome in a matter of days and at low cost. Sequence read lengths, initially very short, have rapidly increased since the technology first appeared, and we now are seeing a growing number of efforts to sequence large genomes de novo from these short reads. In this Perspective, we describe the issues associated with short-read assembly, the different types of data produced by second-gen sequencers, and the latest assembly algorithms designed for these data. We also review the genomes that have been assembled recently from short reads and make recommendations for sequencing strategies that will yield a high-quality assembly.

[PubMed - indexed for MEDLINE]
Free PMC Article

Images from this publication.See all images (3)Free text

Figure 1.
Figure 2.
Figure 3.
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk