Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
EMBO J. 1991 Jul;10(7):1833-42.

A pause site for RNA polymerase II is associated with termination of transcription.

Author information

  • 1Sir William Dunn School of Pathology, University of Oxford, UK.

Abstract

Termination of transcription by RNA polymerase II has been postulated to involve a pausing process. We have identified such a pause signal, 350 bp into the 3' flanking region of the human alpha 2 globin gene at a position where termination is thought to occur. We show that this pause signal enhances the utilization of an upstream poly(A) site which is otherwise out-competed by a stronger downstream poly(A) site. We also demonstrate that the pause site rescues a poly(A) site that is inactive due to its location within an intron. Using nuclear run-on analysis we show that elongating RNA polymerase II molecules accumulate over this pause signal. Furthermore we show that when the pause site is positioned immediately downstream of a strong poly(A) signal, significant levels of transcription termination take place.

PMID:
2050120
[PubMed - indexed for MEDLINE]
PMCID:
PMC452858
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Loading ...
    Write to the Help Desk