Biochem Biophys Res Commun. 1991 Jun 14;177(2):659-63.

Expression in monkey cells of the missing enzyme in L-ascorbic acid biosynthesis, L-gulono-gamma-lactone oxidase.

Yagi K, Koshizaka T, Kito M, Ozawa T, Nishikimi M.

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Institute of Applied Biochemistry, Yagi Memorial Park, Japan.

Abstract

L-Gulono-gamma-lactone oxidase (GLO), an enzyme that is missing in scurvy-prone animals, was produced in COS-1 cells by transfection with a minigene constructed from pSVL vector and rat GLO cDNA. A functional GLO protein was expressed, which was indistinguishable from rat GLO in molecular size. The microsomes obtained from the transfected COS-1 cells showed GLO activity comparable to that in rat liver microsomes.

PMID:: 2049088; [PubMed - indexed for MEDLINE]

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A missense mutation of L-gulono-gamma-lactone oxidase causes the inability of scurvy-prone osteogenic disorder rats to synthesize L-ascorbic acid. [J Biol Chem. 1992]
A missense mutation of L-gulono-gamma-lactone oxidase causes the inability of scurvy-prone osteogenic disorder rats to synthesize L-ascorbic acid.
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Transgenic expression of L-gulono-gamma-lactone oxidase in medaka (Oryzias latipes), a teleost fish that lacks this enzyme necessary for L-ascorbic acid biosynthesis.
Toyohara H, Nakata T, Touhata K, Hashimoto H, Kinoshita M, Sakaguchi M, Nishikimi M, Yagi K, Wakamatsu Y, Ozato K. Biochem Biophys Res Commun. 1996 Jun 25; 223(3):650-3.
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Expression of rat gene for L-gulono-gamma-lactone oxidase, the key enzyme of L-ascorbic acid biosynthesis, in guinea pig cells and in teleost fish rainbow trout (Oncorhynchus mykiss).
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Review Molecular basis for the deficiency in humans of gulonolactone oxidase, a key enzyme for ascorbic acid biosynthesis. [Am J Clin Nutr. 1991]
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Nishikimi M, Yagi K. Am J Clin Nutr. 1991 Dec; 54(6 Suppl):1203S-1208S.
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Review Expression of recombinant L-gulono-gamma-lactone oxidase.
Yagi K, Nishikimi M. Methods Enzymol. 1997; 279:24-9.

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