Scatter plot demonstrating that the relationship between Freezing Duration (log-scaled, residualized for age and z-scored) and mean FDG values at time-2 from the functionally defined BNST Region (residualized for age and z-scored; p<.005 two-tailed uncorrected, yellow in Fig 1c and brain-overlay; standardized and residualized for age). These values represent a replication of the relationship between BNST Region metabolism and freezing duration previously reported in Kalin et al. (2005) across the OFC-lesioned animals and their cage-mate controls.

(a) Five animals received lesions to the OFC (Walker Areas 11, 12, 13 and 14; these lesions are discussed in detail in Kalin et al., 2007). (b) Bar-plot with individual data-points representing a lesion/no lesion by time-1/time-2 interaction for freezing duration assessed in the no eye contact condition during FDG-uptake (see text and supplementary materials for details on this analysis). Details on the extreme outlier in the control group at time 1 (circled) can be found in the supplementary materials. (c) Results from a corresponding voxelwise analysis of brain activation, in which we observed a lesion/no lesion by time-1/time-2 interaction (p<.05 two-tailed uncorrected) and a significant difference at time-2 (p<.05 and p<.005, two-tailed uncorrected; seen in red and yellow respectively). Results are overlaid on a study specific template discussed in Kalin et al. (2005), and shown next to a schematic that demonstrates the location of the Bed Nucleus of Stria Terminalis (BNST) region according to Paxinos, Huang and Toga (2000). (d) Bar-plot with individual datapoints representing the lesion/no lesion by time-1/time-2 interaction for the mean-FDG values within the BNST-region cluster (shown in yellow Fig1c).