A novel mechanism for neutrophil priming in trauma: potential role of peritoneal fluid

Surgery. 2010 Aug;148(2):263-70. doi: 10.1016/j.surg.2010.03.019. Epub 2010 May 13.

Abstract

Background: We sought to determine the effect of peritoneal fluid from a novel animal model of abdominal compartment syndrome (ACS) on the proinflammatory status of polymorphonuclear leukocytes (PMNs) and monocytes. We hypothesize that peritoneal fluid is a potential priming and/or activating agent for PMNs/monocytes.

Methods: ACS was induced in female Yorkshire swine, and peritoneal fluid was collected at the time of decompressive laparotomy. Naïve PMNs/monocytes were primed and/or activated with peritoneal fluid, phosphatidylcholine (PAF) plus peritoneal fluid, peritoneal fluid plus n-formyl-met-leu-phe (fMLP), and peritoneal fluid plus phorbol 12-myristate 13-acetate (PMA). Activation was determined by surface marker expression of integrins (CD11b an CD18) and selectins (CD62L). Additionally, proinflammatory cytokines in peritoneal fluid were analyzed.

Results: Peritoneal fluid did not activate PMNs but increased CD11b expression on monocytes. When used as a primer for fMLP- or PMA-induced activation, peritoneal fluid significantly increased CD11b and CD18 expression on PMNs and monocytes. Peritoneal fluid collected at 6 and 12 h post decompressive laparotomy had similar effects. Interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) levels were increased in peritoneal fluid.

Conclusion: Peritoneal fluid represents a primer for PMNs/monocytes and seems to act through receptor-dependent and receptor-independent pathways. Strategies to reduce the amount of peritoneal fluid may decrease the locoregional and systemic inflammatory response by reducing priming and activation of neutrophils/monocytes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ascitic Fluid / physiology*
  • Compartment Syndromes / etiology
  • Compartment Syndromes / physiopathology*
  • Compartment Syndromes / surgery
  • Cytokines / physiology
  • Decompression, Surgical
  • Disease Models, Animal
  • Female
  • In Vitro Techniques
  • Inflammation Mediators / physiology
  • Interleukin-6 / physiology
  • Models, Biological
  • Monocytes / physiology
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Neutrophil Activation / drug effects
  • Neutrophil Activation / physiology
  • Neutrophils / drug effects
  • Neutrophils / physiology*
  • Resuscitation / adverse effects
  • Sus scrofa
  • Tumor Necrosis Factor-alpha / physiology
  • Wounds and Injuries / complications
  • Wounds and Injuries / physiopathology*

Substances

  • Cytokines
  • Inflammation Mediators
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • N-Formylmethionine Leucyl-Phenylalanine