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J Struct Biol. 2010 Oct;172(1):107-19. doi: 10.1016/j.jsb.2010.05.005. Epub 2010 May 11.

Biophysical characterization of recombinant proteins: a key to higher structural genomics success.

Author information

  • 1Structural Genomics Consortium, University of Toronto, Room 839, MaRS Center, South Tower, Toronto, Ontario, Canada. mvedadi@uhnres.utoronto.ca

Abstract

Hundreds of genomes have been successfully sequenced to date, and the data are publicly available. At the same time, the advances in large-scale expression and purification of recombinant proteins have paved the way for structural genomics efforts. Frequently, however, little is known about newly expressed proteins calling for large-scale protein characterization to better understand their biochemical roles and to enable structure-function relationship studies. In the Structural Genomics Consortium (SGC), we have established a platform to characterize large numbers of purified proteins. This includes screening for ligands, enzyme assays, peptide arrays and peptide displacement in a 384-well format. In this review, we describe this platform in more detail and report on how our approach significantly increases the success rate for structure determination. Coupled with high-resolution X-ray crystallography and structure-guided methods, this platform can also be used toward the development of chemical probes through screening families of proteins against a variety of chemical series and focused chemical libraries.

PMID:
20466062
[PubMed - indexed for MEDLINE]
PMCID:
PMC2954336
Free PMC Article

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