Molecular structure of RhCG. (A) Cytoplasmic view of the RhCG with monomer in cartoon representation and complete symmetry-generated biological molecule as ribbon. Threefold symmetry axis indicated by ▴, and • indicates the channel position of each monomer. Transmembrane helices are progressively colored blue to red, indicating succession from N to C terminus. (B) Cytoplasmic molecular surface of RhCG colored by electrostatic potential (19) reveals channel apertures surrounded by negative charge (red) with positive charge (blue) at the periphery. (C and D) Representation of A and B, respectively, rotated 90° in membrane (gray) cross-section with cytoplasmic surface up.

The “M0 lock” in RhCG. (A) Cartoon representation of RhCG rendered in PyMol (20). The side chains of Q19 on M0 and Q101 on M2 are shown as stick representation colored by atom type. The hydrogen bonding between the side chains are indicted by dashed lines. (B) Closer view of the M0 lock with all side chains displayed as sticks and colored by atom type (C: white; O: red; N: blue; S: yellow).

RhCG NH₃ channel and shunt. Partially transparent surface generated by HOLLOW (22) of the channel, voids, and shunt. Select side chains that compose this surface are displayed as sticks. Water molecules within the channel surface displayed as red spheres. All atoms colored by atom type with carbons in white except for the shunt with carbons in green.

Channel NH₃ transport in proteoliposomes. (A) Relative vesicular pH change indicated by CF fluorescence for RhCG at protein to lipid ratio 1∶90 by weight (open blue triangles) and 1∶45 (closed blue triangles) demonstrated protein dependent NH₃ permeability relative to empty liposomes (black squares) and a control protein YaaH (red diamonds). (B) Proteoliposomes from the same sample preparations after addition of 275 mM sucrose at t = 0 were monitored for transport of water resulting in decreased vesicular volume, increased CF concentration, and subsequent self-quenching. Data displayed for the exponential fluorescence decay after lag time to reach self-quenching concentration and exponential fit (black lines).

Phenylalanine gate and polar cluster P3. The partially transparent channel surface and nearby void surfaces are displayed to reveal the waters with observed electron density in the molecular structure. The side chains of Phe gate and P3 residues are shown as sticks colored by atom type. The P3 cluster for each monomer (structure model in white) involves Y254 from the adjacent monomer (labeled with * and in blue).

Proposed mechanism of acid/ammonium balance in the epithelium of the acid-secreting renal intercalated cells. Channels and pumps are indicated as tubes through the membrane. Substrates are indicated as atomic models. The net flow of respective substrates and diffusion through the membrane are indicated by arrows. Figure is based on the proposed epithelial model for acid-secreting intercalated cells (7, 47, 48).