J Chromatogr. 1991 Feb 22;539(2):511-6.

Purification on poly(U)-sepharose 4B of human breast cancer cell line T-47D DNA polymerases.

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Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, Clermont-Ferrand, France.

Abstract

The purification of DNA polymerases (RNA-directed DNA polymerases and DNA-directed DNA polymerases) on poly(U)-Sepharose 4B from a breast tumour cell line (T-47D) is reported. The elution of these enzymes was followed in each fraction by activity measurements with the four primer-templates poly(rA)-oligo(dT)12-18, poly(dA) oligo(dT)12-18, poly(rC)-oligo(dG)12-18 and poly(rCm)-oligo (dG)12-18. The control of the polymerase purification by chromatography was performed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis of the pooled active enzymatic fractions.

PMID:: 2045460; [PubMed - indexed for MEDLINE]

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