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PLoS One. 2010 Apr 28;5(4):e10391. doi: 10.1371/journal.pone.0010391.

Formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.

Author information

  • 1Max Planck Institute of Psychiatry, Munich, Germany. Ulrich.leischner@lrz.tum.de

Abstract

Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front of a bright background. Here, we show this effect on a three-dimensional image stack of a hippocampus sample, focusing on the CA1 region. This method, called FIF-Ultramicroscopy, allows for the three-dimensional observation of cellular structures in various tissue types without complicated staining techniques.

PMID:
20436930
[PubMed - indexed for MEDLINE]
PMCID:
PMC2861007
Free PMC Article

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