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Appl Environ Microbiol. 2010 Jul;76(13):4571-3. doi: 10.1128/AEM.02825-09. Epub 2010 Apr 30.

Practical method for extraction of PCR-quality DNA from environmental soil samples.

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  • 1Centers for Disease Control and Prevention, MS G-13, 1600 Clifton Rd., Atlanta, GA 30333, USA.

Abstract

Methods for the extraction of PCR-quality DNA from environmental soil samples by using pairs of commercially available kits were evaluated. Coxiella burnetii DNA was detected in spiked soil samples at <1,000 genome equivalents per gram of soil and in 12 (16.4%) of 73 environmental soil samples.

PMID:
20435765
[PubMed - indexed for MEDLINE]
PMCID:
PMC2897455
Free PMC Article
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