DNA methylation profiling using HpaII tiny fragment enrichment by ligation-mediated PCR (HELP)

Masako Suzuki; John M Greally

doi:10.1016/j.ymeth.2010.04.013

DNA methylation profiling using HpaII tiny fragment enrichment by ligation-mediated PCR (HELP)

Methods. 2010 Nov;52(3):218-22. doi: 10.1016/j.ymeth.2010.04.013. Epub 2010 Apr 29.

Authors

Masako Suzuki¹, John M Greally

Affiliation

¹ Center for Epigenomics and Division of Computational Genetics, Department of Genetics, Albert Einstein College of Medicine, 1301 Morris Park Avenue, Bronx, NY 10461, USA.

Abstract

The HELP assay is a technique that allows genome-wide analysis of cytosine methylation. Here we describe the assay, its relative strengths and weaknesses, and the transition of the assay from a microarray to massively-parallel sequencing-based foundation.

Publication types

Review

MeSH terms

Animals
DNA / chemistry
DNA / genetics
DNA / metabolism*
DNA Methylation*
Deoxyribonuclease HpaII / metabolism*
Humans
Polymerase Chain Reaction / methods*

Substances

DNA
Deoxyribonuclease HpaII

Grants and funding

R01 HG004401/HG/NHGRI NIH HHS/United States