Methods. 2010 Nov;52(3):218-22. Epub 2010 Apr 29.

DNA methylation profiling using HpaII tiny fragment enrichment by ligation-mediated PCR (HELP).

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Center for Epigenomics and Division of Computational Genetics, Department of Genetics, Albert Einstein College of Medicine, 1301 Morris Park Avenue, Bronx, NY 10461, USA.

Abstract

The HELP assay is a technique that allows genome-wide analysis of cytosine methylation. Here we describe the assay, its relative strengths and weaknesses, and the transition of the assay from a microarray to massively-parallel sequencing-based foundation.

PMID:: 20434563; [PubMed - indexed for MEDLINE]
PMCID:: PMC2954265

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