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BMC Infect Dis. 2010 Apr 29;10:104. doi: 10.1186/1471-2334-10-104.

The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assays.

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  • 1Laboratory Bacteriology Research, Department of Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium.

Abstract

BACKGROUND:

Streptococcus pneumoniae is one of the most frequently encountered pathogens in humans but its differentiation from closely related but less pathogenic streptococci remains a challenge.

METHODS:

This report describes a newly-developed PCR assay (Spne-PCR), amplifying a 217 bp product of the 16S rRNA gene of S. pneumoniae, and its performance compared to other genotypic and phenotypic tests.

RESULTS:

The new PCR assay designed in this study, proved to be specific at 57 degrees C for S. pneumoniae, not amplifying S. pseudopneumoniae or any other streptococcal strain or any strains from other upper airway pathogenic species. PCR assays (psaA, LytA, ply, spn9802-PCR) were previously described for the specific amplification of S. pneumoniae, but psaA-PCR was the only one found not to cross-react with S. pseudopneumoniae.

CONCLUSION:

Spne-PCR, developed for this study, and psaA-PCR were the only two assays which did not mis-identify S. pseudopneumoniae as S. pneumoniae. Four other PCR assays and the AccuProbe assay were unable to distinguish between these species.

PMID:
20426878
[PubMed - indexed for MEDLINE]
PMCID:
PMC2874796
Free PMC Article

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