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Biochem Biophys Res Commun. 2010 May 28;396(2):457-62. doi: 10.1016/j.bbrc.2010.04.116. Epub 2010 Apr 24.

Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells.

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  • 1National Institute of Cellular Biotechnology, Dublin City University, Dublin 9, Ireland. Erica.hennessy4@mail.dcu.ie

Abstract

MicroRNAs (miRNAs) are a family of endogenous small non-coding RNAs which regulate mRNAs at the post-transcriptional level. MiRNAs have been identified in both normal physiological and pathological conditions. To date, a limited number of miRNAs have been shown to be involved in the regulation of insulin secretion. We have identified a panel of 10 miRNAs down-regulated in glucose non-responsive MIN6 cells compared to glucose responsive cells using TaqMan Low Density miRNA Arrays. Of these 10 miRNA targets, subsequent functional investigations involving knockdown of mir-200a, mir-130a and mir-410 levels suggested that they may decrease the capability of MIN6 cells to secrete insulin in response to stimulatory levels of glucose. Conversely, experiment with over-expression of mir-410 suggest that it may enhance levels of glucose stimulated insulin secretion. In this study, we have also identified 21 miRNAs not previously known to have a potential murine homologue.

Copyright (c) 2010 Elsevier Inc. All rights reserved.

PMID:
20417623
[PubMed - indexed for MEDLINE]
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