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    Vaccine. 2010 Jun 7;28(25):4145-52. Epub 2010 Apr 22.

    Soluble recombinant human metapneumovirus G protein is immunogenic but not protective.

    Ryder AB, Tollefson SJ, Podsiad AB, Johnson JE, Williams JV.

    Source

    Vanderbilt University Medical Center, School of Medicine, Nashville, TN 37232-2581, USA.

    Abstract

    Human metapneumovirus (HMPV) expresses the major surface glycoproteins F and G. We evaluated the protective efficacy of immunization with G. We generated a recombinant form of G ectodomain (GDeltaTM) that was secreted from mammalian cells and purified by affinity chromatography. We tested the immunogenicity of GDeltaTM in cotton rats. Animals were immunized with PBS, GDeltaTM alone or adjuvanted, or were infected once with HMPV, and challenged with live HMPV at 28 days. Animals vaccinated with adjuvanted and non-adjuvanted GDeltaTM developed high levels of serum antibodies to both recombinant and native G protein; however, vaccinated animals did not develop neutralizing antibodies and were not protected against virus challenge. Unlike the analogous non-fusion glycoproteins of other human paramyxoviruses, HMPV G does not appear to be a protective antigen. This represents an unusual feature of HMPV.

    Copyright 2010 Elsevier Ltd. All rights reserved.

    PMID:
    20417260
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC2894472
    Free PMC Article

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