(A) Schematic representation of the reaction and the compounds consumed and generated and designation of the positions followed by NMR. (B) Region of interest from the 1H-NMR spectra of the reaction of Rv1248c with α-KG in the presence of GLX. The spectra were recorded every 4 min for ~ 2 hours. Reaction mix contained 50 mM KPi, pH 7.4, 2 mM MgCl2, 300 µM TDP, 10 mM α-KG, 10 mM GLX and 500 nM Rv1248c. Resonance 5 cannot be seen as it overlaps with the water signal. (C) Region of interest from the 1H-NMR spectra of the reaction of Rv1248c with α-KG. Reaction mix contained 50 mM KPi, pH 7.4, 2 mM MgCl2, 300 µM TDP, 10 mM α-KG and 500 nM Rv1248c. (D) Stack plot of the region of interest from the 1H-NMR spectra of the reaction of Rv1248c with α-KG and GLX. Spectra were recorded every 10 min for ~ 14 hours. Reaction mix contained 50 mM KPi, pH 7.4, 2 mM MgCl2, 100 µM TDP, 2 mM α-KG, 2 mM GLX and 500 nM Rv1248c.