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Science. 2010 May 14;328(5980):912-6. doi: 10.1126/science.1185880. Epub 2010 Apr 22.

Small RNA duplexes function as mobile silencing signals between plant cells.

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  • 1Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg 12 rue du Général Zimmer, 67084 Strasbourg cedex, France. patrice.dunoyer@ibmp-ulp.u-strasbg.fr

Erratum in

  • Science. 2010 Jun 4;328(5983):1229.
  • Science. 2011 Apr 15;332(6027):306.

Abstract

In the plant RNA interference (RNAi) pathway, 21-nucleotide duplexes of small interfering RNA (siRNA) are processed from longer double-stranded RNA precursors by the RNaseIII Dicer-like 4 (DCL4). Single-stranded siRNAs then guide Argonaute 1 (AGO1) to execute posttranscriptional silencing of complementary target RNAs. RNAi is not cell-autonomous in higher plants, but the nature of the mobile nucleic acid(s) signal remains unknown. Using cell-specific rescue of DCL4 function and cell-specific inhibition of RNAi movement, we genetically establish that exogenous and endogenous siRNAs, as opposed to their precursor molecules, act as mobile silencing signals between plant cells. We further demonstrate physical movement of mechanically delivered, labeled siRNA duplexes that functionally recapitulate transgenic RNAi spread. Cell-to-cell movement is unlikely to involve AGO1-bound siRNA single strands, but instead likely involves siRNA duplexes.

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PMID:
20413458
[PubMed - indexed for MEDLINE]
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