Clin Microbiol Infect. 2011 Feb;17(2):241-4. doi: 10.1111/j.1469-0691.2010.03246.x.

Direct pathogen detection from swab samples using a new high-throughput sequencing technology.

Yongfeng H, Fan Y, Jie D, Jian Y, Ting Z, Lilian S, Jin Q.

Source

State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, China.

Abstract

The detection of emerging infectious diseases has been a continuing concern, especially with the novel influenza A (H1N1) viral pandemic of 2009. In the present study, we validated a 'second-generation' parallel sequencing platform for viral detection in swab samples collected during recent influenza virus infections in Beijing. This operation yielded millions of valid reads per sample and resulted in an almost complete spectrum of nucleotide information. Importantly, novel A (H1N1) and seasonal A (H3N2) influenza virus-derived sequences were detected without prior knowledge or use of genetic information in advance, suggesting that this approach could be a valuable tool for diagnosing emerging infectious diseases.

PMID:: 20412188; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

LinkOut - more resources

Full Text Sources

Medical

Flu - MedlinePlus Health Information

Supplemental Content

Related citations

Performance testing of two new one-step real time PCR assays for detection of human influenza and avian influenza viruses isolated in humans and respiratory syncytial virus. [J Prev Med Hyg. 2006]
Performance testing of two new one-step real time PCR assays for detection of human influenza and avian influenza viruses isolated in humans and respiratory syncytial virus.
Valle L, Amicizia D, Bacilieri S, Banfi F, Riente R, Durando P, Sticchi L, Gasparini R, Esposito C, Icardi G, et al. J Prev Med Hyg. 2006 Dec; 47(4):127-33.
Initial identification and characterization of an emerging zoonotic influenza virus prior to pandemic spread. [J Clin Microbiol. 2010]
Initial identification and characterization of an emerging zoonotic influenza virus prior to pandemic spread.
Metzgar D, Baynes D, Myers CA, Kammerer P, Unabia M, Faix DJ, Blair PJ. J Clin Microbiol. 2010 Nov; 48(11):4228-34. Epub 2010 Sep 22.
Detection of pandemic influenza A H1N1 virus by multiplex reverse transcription-PCR with a GeXP analyzer. [J Virol Methods. 2010]
Detection of pandemic influenza A H1N1 virus by multiplex reverse transcription-PCR with a GeXP analyzer.
Qin M, Wang DY, Huang F, Nie K, Qu M, Wang M, Shu YL, Ma XJ. J Virol Methods. 2010 Sep; 168(1-2):255-8. Epub 2010 May 7.
Review The application of genomics to emerging zoonotic viral diseases. [PLoS Pathog. 2009]
Review The application of genomics to emerging zoonotic viral diseases.
Haagmans BL, Andeweg AC, Osterhaus AD. PLoS Pathog. 2009 Oct; 5(10):e1000557. Epub 2009 Oct 26.
Review Methods for molecular surveillance of influenza. [Expert Rev Anti Infect Ther. 2010]
Review Methods for molecular surveillance of influenza.
Wang R, Taubenberger JK. Expert Rev Anti Infect Ther. 2010 May; 8(5):517-27.

See reviews... See all...

Cited by 1 PubMed Central article

Applications of next-generation sequencing technologies to diagnostic virology. [Int J Mol Sci. 2011]
Applications of next-generation sequencing technologies to diagnostic virology.
Barzon L, Lavezzo E, Militello V, Toppo S, Palù G. Int J Mol Sci. 2011; 12(11):7861-84. Epub 2011 Nov 14.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Direct pathogen detection from swab samples using a new high-throughput sequenci...
Direct pathogen detection from swab samples using a new high-throughput sequencing technology.
Clin Microbiol Infect. 2011 Feb ;17(2):241-4. doi: 10.1111/j.1469-0691.2010.03246.x.

PubMed
Characterization of transcriptional complexity during berry development in Vitis...
Characterization of transcriptional complexity during berry development in Vitis vinifera using RNA-Seq.
Plant Physiol. 2010 Apr ;152(4):1787-95. Epub 2010 Jan 29 .

PubMed
Transcriptome response to pollutants and insecticides in the dengue vector Aedes...
Transcriptome response to pollutants and insecticides in the dengue vector Aedes aegypti using next-generation sequencing technology.
BMC Genomics. 2010 Mar 31 ;11:216.

PubMed
Emotional attribution in high-functioning individuals with autistic spectrum dis...
Emotional attribution in high-functioning individuals with autistic spectrum disorder: a functional imaging study.
J Am Acad Child Adolesc Psychiatry. 2004 Apr ;43(4):473-80.

PubMed
Facilitation of task performance and removal of the effects of sleep deprivation...
Facilitation of task performance and removal of the effects of sleep deprivation by an ampakine (CX717) in nonhuman primates.
PLoS Biol. 2005 Sep ;3(9):e299. Epub 2005 Aug 23 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: