Somatic Cell Genet. 1978 Jan;4(1):1-12.

Adenosine kinase as a new selective marker in somatic cell genetics: isolation of adenosine kinase--deficient mouse cell lines and human--mouse hybrid cell lines containing adenosine kinase.

Abstract

A new selective system for isolating somatic cell hybrids, using adenosine kinase as the selective marker, has been developed. The selective medium for forward selection (to select for cells containing adenosine kinase) contains alanosine, adenosine and uridine. To survive in the presence of alanosine, cells must have adenosine kinase in order to utilize exogenous adenosine as the sole source of AMP. Uridine is added to the selective medium to prevent the toxic effects of adenosine on cultured mammalian cells. The selective medium for reverse selection (to select for cells lacking adenosine kinase) contains 2-fluoroadenosine, an analogue of adenosine, which is converted to a toxic nucleotide by the action of adenosine kinase. Mouse mutant cell lines deficient in adenosine kinase have been derived. Human--mouse hybrid cells containing the kinase have been prepared from one of these mutant lines. Karyotype data of these hygrid lines and their adenosine kinase-minus sublines are consistent with assignment by others of the human gene for adenosine kinase on chromosome 10.

PMID:: 204068; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Molecular Biology Databases

Miscellaneous

Mouse Genome Informatics (MGI)

Supplemental Content

Save items

Related citations in PubMed

Assignment of the gene for adenosine kinase to chromosome 14 in Mus musculus by somatic cell hybridization. [Cytogenet Cell Genet. 1978]
Assignment of the gene for adenosine kinase to chromosome 14 in Mus musculus by somatic cell hybridization.
Leinwand L, Fournier RE, Nichols EA, Ruddle FH. Cytogenet Cell Genet. 1978; 21(1-2):77-85.
Human-mouse somatic cell hybrid lines selected for human adenosine kinase: a new selective method. [Birth Defects Orig Artic Ser. ...]
Human-mouse somatic cell hybrid lines selected for human adenosine kinase: a new selective method.
Chan TS, Creagan RP. Birth Defects Orig Artic Ser. 1976; 12(7):378-81.
A human-mouse somatic hybrid line selected for human deoxycytidine deaminase. [Somatic Cell Genet. 1975]
A human-mouse somatic hybrid line selected for human deoxycytidine deaminase.
Chan TS, Long C, Green H. Somatic Cell Genet. 1975 Jan; 1(1):81-90.
Increased expression of one of two adenosine deaminase alleles in a human choriocarcinoma cell line following selection with adenine nucleosides. [J Biol Chem. 1983]
Increased expression of one of two adenosine deaminase alleles in a human choriocarcinoma cell line following selection with adenine nucleosides.
Yeung CY, Riser ME, Kellems RE, Siciliano MJ. J Biol Chem. 1983 Jul 10; 258(13):8330-7.
Human-mouse somatic cell hybrid lines selected for human adenosine kinase: a new selective method. [Cytogenet Cell Genet. 1976]
Human-mouse somatic cell hybrid lines selected for human adenosine kinase: a new selective method.
Chan TS, Creagan RP. Cytogenet Cell Genet. 1976; 16(1-5):378-81.

See reviews... See all...

Cited by 3 PubMed Central articles

Chromosome segregation is frequently associated with the expression of recessive mutations in mouse cells. [Proc Natl Acad Sci U S A. 1981]
Chromosome segregation is frequently associated with the expression of recessive mutations in mouse cells.
Eves EM, Farber RA. Proc Natl Acad Sci U S A. 1981 Mar; 78(3):1768-72.
Selection and amplification of heterologous genes encoding adenosine deaminase in mammalian cells. [Proc Natl Acad Sci U S A. 1986]
Selection and amplification of heterologous genes encoding adenosine deaminase in mammalian cells.
Kaufman RJ, Murtha P, Ingolia DE, Yeung CY, Kellems RE. Proc Natl Acad Sci U S A. 1986 May; 83(10):3136-40.
Purine excretion by mouse peritoneal macrophages lacking adenosine deaminase activity. [Proc Natl Acad Sci U S A. 1979]
Purine excretion by mouse peritoneal macrophages lacking adenosine deaminase activity.
Chan TS. Proc Natl Acad Sci U S A. 1979 Feb; 76(2):925-9.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound
PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (OMIM)
Gene records associated with Online Mendelian Inheritance in Man (OMIM) records that cite the current articles in their reference lists.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
OMIM (calculated)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as references in the light bulb links within or in the citations at the end of the OMIM record. The references available through the light bulb link are collected using the PubMed related articles algorithm to identify records with similar terminology to the OMIM record.
OMIM (cited)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as reference cited at the end of the OMIM record.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance
PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Adenosine kinase as a new selective marker in somatic cell genetics: isolation o...
Adenosine kinase as a new selective marker in somatic cell genetics: isolation of adenosine kinase--deficient mouse cell lines and human--mouse hybrid cell lines containing adenosine kinase.
Somatic Cell Genet. 1978 Jan ;4(1):1-12.

PubMed
HIV transmission risk through anal intercourse: systematic review, meta-analysis...
HIV transmission risk through anal intercourse: systematic review, meta-analysis and implications for HIV prevention.
Int J Epidemiol. 2010 Aug ;39(4):1048-63. Epub 2010 Apr 20 .

PubMed
Chronic kidney disease bone and mineral disorder (CKD-MBD) in apolipoprotein E-d...
Chronic kidney disease bone and mineral disorder (CKD-MBD) in apolipoprotein E-deficient mice with chronic renal failure.
Bone. 2010 Jul ;47(1):156-63. Epub 2010 Apr 18 .

PubMed
Combined treatment with MAO-A inhibitor and MAO-B inhibitor increases extracellu...
Combined treatment with MAO-A inhibitor and MAO-B inhibitor increases extracellular noradrenaline levels more than MAO-A inhibitor alone through increases in beta-phenylethylamine.
Eur J Pharmacol. 2010 Jul 10 ;637(1-3):77-82. Epub 2010 Apr 18 .

PubMed
Polymorphisms in Gag spacer peptide 1 confer varying levels of resistance to the...
Polymorphisms in Gag spacer peptide 1 confer varying levels of resistance to the HIV- 1 maturation inhibitor bevirimat.
Retrovirology. 2010 Apr 20 ;7:36.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Adenosine kinase as a new selective marker in somatic cell genetics: isolation of adenosine kinase--deficient mouse cell lines and human--mouse hybrid cell lines containing adenosine kinase.

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Molecular Biology Databases

Miscellaneous

Supplemental Content

Save items

Related citations in PubMed

Cited by 3 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information