Protein complexes/aggregates as potential cancer biomarkers revealed by a nanoparticle aggregation immunoassay

Colloids Surf B Biointerfaces. 2010 Jul 1;78(2):259-65. doi: 10.1016/j.colsurfb.2010.03.012. Epub 2010 Mar 25.

Abstract

Protein-protein interactions and protein complex/aggregate formation play an essential role in almost all biological functions and activities. Through a nanoparticle aggregation immunoassay, we discovered that some proteins are substantially more complexed/aggregated in cancer tissues than normal tissues. This study examined four biomarkers proteins, CA125, CEA (carcinoembryonic antigen), CA19-9 and PAP (prostatic acid phosphatase) in ovarian, colon and prostate tissue lysates. The most exciting results were observed from the PAP assay of prostate tissues: prostate cancer can be clearly distinguished from normal prostate and prostate with benign conditions such as BPH (benign prostate hyperplasia) based on the complex/aggregation level of PAP in prostate tissue lysates. The complex/aggregate level of a protein can be potential biomarkers for cancer detection and diagnosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Phosphatase
  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies / chemistry
  • Biomarkers, Tumor / analysis*
  • Biomarkers, Tumor / chemistry
  • CA-125 Antigen / analysis
  • CA-125 Antigen / chemistry
  • CA-19-9 Antigen / analysis
  • CA-19-9 Antigen / chemistry
  • Carcinoembryonic Antigen / analysis
  • Carcinoembryonic Antigen / chemistry
  • Colonic Neoplasms / diagnosis
  • Colonic Neoplasms / metabolism
  • Diagnosis, Differential
  • Female
  • Gold / chemistry
  • Humans
  • Immunoassay / methods*
  • Male
  • Membrane Proteins / analysis
  • Membrane Proteins / chemistry
  • Metal Nanoparticles / chemistry*
  • Middle Aged
  • Neoplasms / diagnosis
  • Neoplasms / metabolism*
  • Ovarian Neoplasms / diagnosis
  • Ovarian Neoplasms / metabolism
  • Prostatic Hyperplasia / diagnosis
  • Prostatic Hyperplasia / metabolism
  • Prostatic Neoplasms / diagnosis
  • Prostatic Neoplasms / metabolism
  • Protein Binding
  • Protein Conformation
  • Protein Tyrosine Phosphatases / analysis
  • Protein Tyrosine Phosphatases / chemistry
  • Proteins / analysis*
  • Proteins / chemistry
  • Sensitivity and Specificity

Substances

  • Antibodies
  • Biomarkers, Tumor
  • CA-125 Antigen
  • CA-19-9 Antigen
  • Carcinoembryonic Antigen
  • MUC16 protein, human
  • Membrane Proteins
  • Proteins
  • Gold
  • Acid Phosphatase
  • prostatic acid phosphatase
  • Protein Tyrosine Phosphatases