Abstract
Protein-protein interactions and protein complex/aggregate formation play an essential role in almost all biological functions and activities. Through a nanoparticle aggregation immunoassay, we discovered that some proteins are substantially more complexed/aggregated in cancer tissues than normal tissues. This study examined four biomarkers proteins, CA125, CEA (carcinoembryonic antigen), CA19-9 and PAP (prostatic acid phosphatase) in ovarian, colon and prostate tissue lysates. The most exciting results were observed from the PAP assay of prostate tissues: prostate cancer can be clearly distinguished from normal prostate and prostate with benign conditions such as BPH (benign prostate hyperplasia) based on the complex/aggregation level of PAP in prostate tissue lysates. The complex/aggregate level of a protein can be potential biomarkers for cancer detection and diagnosis.
2010 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acid Phosphatase
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Adult
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Aged
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Aged, 80 and over
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Antibodies / chemistry
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Biomarkers, Tumor / analysis*
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Biomarkers, Tumor / chemistry
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CA-125 Antigen / analysis
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CA-125 Antigen / chemistry
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CA-19-9 Antigen / analysis
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CA-19-9 Antigen / chemistry
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Carcinoembryonic Antigen / analysis
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Carcinoembryonic Antigen / chemistry
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Colonic Neoplasms / diagnosis
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Colonic Neoplasms / metabolism
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Diagnosis, Differential
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Female
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Gold / chemistry
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Humans
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Immunoassay / methods*
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Male
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Membrane Proteins / analysis
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Membrane Proteins / chemistry
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Metal Nanoparticles / chemistry*
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Middle Aged
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Neoplasms / diagnosis
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Neoplasms / metabolism*
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Ovarian Neoplasms / diagnosis
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Ovarian Neoplasms / metabolism
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Prostatic Hyperplasia / diagnosis
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Prostatic Hyperplasia / metabolism
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Prostatic Neoplasms / diagnosis
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Prostatic Neoplasms / metabolism
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Protein Binding
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Protein Conformation
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Protein Tyrosine Phosphatases / analysis
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Protein Tyrosine Phosphatases / chemistry
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Proteins / analysis*
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Proteins / chemistry
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Sensitivity and Specificity
Substances
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Antibodies
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Biomarkers, Tumor
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CA-125 Antigen
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CA-19-9 Antigen
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Carcinoembryonic Antigen
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MUC16 protein, human
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Membrane Proteins
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Proteins
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Gold
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Acid Phosphatase
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prostatic acid phosphatase
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Protein Tyrosine Phosphatases