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Lab Chip. 2010 May 7;10(9):1109-12. doi: 10.1039/c001200a. Epub 2010 Mar 19.

Color and monochrome lensless on-chip imaging of Caenorhabditis elegans over a wide field-of-view.

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  • 1Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.

Abstract

We demonstrate color and monochrome on-chip imaging of Caenorhabditis elegans samples over a wide field-of-view using incoherent lensless in-line holography. Digital reconstruction of the recorded lensless holograms rapidly creates the C. elegans images within <1 s over a field-of-view of >24 mm2. By digitally combining the reconstructed images at three different wavelengths (red, green and blue), color images of dyed samples are also acquired. This wide field-of-view and compact on-chip imaging modality also permits straightforward integration with microfluidic systems.

PMID:
20390127
[PubMed - indexed for MEDLINE]
PMCID:
PMC2869489
Free PMC Article
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