Display Settings:

Format

Send to:

Choose Destination
Proc Natl Acad Sci U S A. 2010 Apr 27;107(17):7910-5. doi: 10.1073/pnas.0915163107. Epub 2010 Apr 12.

Hybrid PET-optical imaging using targeted probes.

Author information

  • 1Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. MNahrendorf@mgh.harvard.edu

Abstract

Fusion imaging of radionuclide-based molecular (PET) and structural data [x-ray computed tomography (CT)] has been firmly established. Here we show that optical measurements [fluorescence-mediated tomography (FMT)] show exquisite congruence to radionuclide measurements and that information can be seamlessly integrated and visualized. Using biocompatible nanoparticles as a generic platform (containing a (18)F isotope and a far red fluorochrome), we show good correlations between FMT and PET in probe concentration (r(2) > 0.99) and spatial signal distribution (r(2) > 0.85). Using a mouse model of cancer and different imaging probes to measure tumoral proteases, macrophage content and integrin expression simultaneously, we demonstrate the distinct tumoral locations of probes in multiple channels in vivo. The findings also suggest that FMT can serve as a surrogate modality for the screening and development of radionuclide-based imaging agents.

Comment in

PMID:
20385821
[PubMed - indexed for MEDLINE]
PMCID:
PMC2867879
Free PMC Article

Images from this publication.See all images (6)Free text

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk