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Mol Cell. 2010 Apr 9;38(1):78-88. doi: 10.1016/j.molcel.2010.01.041.

Endoplasmic reticulum stress induces G2 cell-cycle arrest via mRNA translation of the p53 isoform p53/47.

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  • 1INSERM Unité 716, Institut de Génétique Moléculaire, Université Paris 7, Hôpital St. Louis, 27 Rue Juliette Dodu, 75010 Paris, France.

Abstract

p53 downstream pathways control G1 and G2 cell-cycle arrest, DNA repair, or apoptosis. However, it is still not clear how cells differentiate the cell-biological outcome of p53 activation in response to different types of stresses. The p53/47 isoform lacks the first 39 amino acids of full-length p53 including the Mdm2 binding site and the first trans-activation domain, and tetramers including p53/47 exhibit altered activity and biochemical properties. Here we show that endoplasmic reticulum stress promotes PERK-dependent induction of p53/47 mRNA translation and p53/47 homo-oligomerization. p53/47 induces 14-3-3sigma and G2 arrest but does not affect G1 progression. This is contrary to p53FL, which promotes G1 arrest but has no effect on the G2. These results show a unique role for p53/47 in the p53 pathway and illustrate how a cellular stress leads to a defined cell-biological outcome through expression of a p53 isoform.

2010 Elsevier Inc. All rights reserved.

PMID:
20385091
[PubMed - indexed for MEDLINE]
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