In order to test ab initio calculations of light nuclei, we have remeasured lifetimes in 10Be using the Doppler shift attenuation method (DSAM) following the 7Li(7Li,alpha)10Be reaction at 8 and 10 MeV. The new experiments significantly reduce systematic uncertainties in the DSAM technique. The J(pi) = 2(1)(+) state at 3.37 MeV has tau = 205 +/- (5)(stat) +/- (7)(sys) fs corresponding to a B(E2 down) of 9.2(3)e(2) fm(4) in broad agreement with many calculations. The J(pi) = 2(2)(+) state at 5.96 MeV was found to have a B(E2 down) of 0.11(2)e(2) fm(4) and provides a more discriminating test of nuclear models. New Green's function Monte Carlo calculations for these states and transitions with a number of Hamiltonians are also reported and compared to experiment.