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Eukaryot Cell. 2010 May;9(5):831-3. Epub 2010 Apr 2.

Single-step affinity purification for fungal proteomics.

Liu HL, Osmani AH, Ukil L, Son S, Markossian S, Shen KF, Govindaraghavan M, Varadaraj A, Hashmi SB, De Souza CP, Osmani SA.

Source

Department of Molecular Genetics, Ohio State University, 496 W. 12th Ave., Columbus, OH 43210, USA.

Abstract

A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches, which should be widely applicable, are included.

PMID:: 20363899; [PubMed - indexed for MEDLINE]
PMCID: PMC2863959

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Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

Research Support, N.I.H., Extramural

MeSH Terms

Substances

Fungal Proteins

Grant Support

GM 42564/GM/NIGMS NIH HHS/United States

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Eukaryot Cell. 2010 May ;9(5):831-3. Epub 2010 Apr 2 .

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