Biotechniques. 2010 Feb;48(2):135-8.

A micro-incubator for cell and tissue imaging.

Picard C, Hearnden V, Massignani M, Achouri S, Battaglia G, MacNeil S, Donald A.

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Cavendish Laboratory, University of Cambridge, Cambridge, UK. cyril.picard@legi.grenoble-inp.fr

Abstract

A low-cost micro-incubator for imaging dynamic processes in living cells and tissues has been developed. This micro-incubator provides a tunable environment that can be altered to study responses of cell monolayers for several days as well as relatively thick tissue samples and tissue-engineered epithelial tissues in experiments lasting several hours. Samples are contained in a sterile cavity closed by a gas-permeable membrane. The incubator can be positioned in any direction and used on an inverted or upright microscope. Temperature is regulated using a Peltier module controlled by a sensor positioned close to the sample, enabling compensation for any changes in temperature. Rapid changes in a sample's surrounding environment can be achieved due to the fast response of the Peltier module. These features permit monitoring of sample adaptation to induced environmental changes.

PMID:: 20359297; [PubMed - indexed for MEDLINE]

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A micro-incubator for cell and tissue imaging.

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