J Clin Microbiol. 2010 Jun;48(6):2268-70. Epub 2010 Mar 31.

Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures.

Morgan M, Marlowe E, Della-Latta P, Salimnia H, Novak-Weekley S, Wu F, Crystal BS.

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Cedars Sinai Medical Center, Los Angeles, California, USA.

Abstract

A shortened protocol for two peptide nucleic acid fluorescence in situ hybridization (PNA FISH) assays for the detection of Gram-negative bacilli from positive blood cultures was evaluated in a multicenter trial. There was 100% concordance between the two protocols for each assay (368 of 368 and 370 of 370 results) and 99.7% (367 of 368 and 369 of 370 results) agreement with routine laboratory techniques.

PMID:: 20357212; [PubMed - indexed for MEDLINE]
PMCID: PMC2884511

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