Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Int J Radiat Biol. 2010 Apr;86(4):321-8. doi: 10.3109/09553000903564034.

High-throughput antibody-based assays to identify and quantify radiation-responsive protein biomarkers.

Author information

  • 1Center for Radiological Research, College of Physicians & Surgeons, Columbia University, New York, New York 10032, USA.

Abstract

PURPOSE:

After a radiological 'dirty bomb' incident in a major metropolitan center, substantial numbers of people may be exposed to radiation. However, only a fraction of those individuals will need urgent medical attention. Consequently, a rapid screening test is needed to identify those people who require immediate treatment.

MATERIAL AND METHODS:

Ten normal human cell lines were screened by enzyme-linked immunosorbent assay (ELISA) for the expression of a dozen secreted cytokines that have been reported to have changes in protein or mRNA levels at 1, 2, and 3 days after 0-10 Gy irradiation using (137)Cs gamma rays at 0.82 Gy min(-1). After this systematic in vitro screen, we measured changes in the level of a subset of these candidate proteins in plasma from irradiated C57BL/6 mice (n = 3 per group), comparing shams with a single radiation dose (5 Gy X-rays) at 3.7 Gy min(-1) at 6 h after irradiation.

RESULTS:

We identified four cytokine molecules that had altered levels after radiation exposure, one of which, Interleukin (IL) 6, was consistently elevated after irradiation in vitro and in vivo.

CONCLUSIONS:

Our findings underscore the potential for IL6 as a marker for an immunoassay-based, rapid, high-throughput biodosimeter.

PMID:
20353341
[PubMed - indexed for MEDLINE]
PMCID:
PMC3664638
Free PMC Article

Images from this publication.See all images (2)Free text

Figure 1
Figure 2
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Informa Healthcare Icon for PubMed Central
    Loading ...
    Write to the Help Desk