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Biophys J. 2010 Jan 20;98(2):350-7. doi: 10.1016/j.bpj.2009.10.008.

Analytical method for the fast time-domain reconstruction of fluorescent inclusions in vitro and in vivo.

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  • 1Department of Radiology and Moores UCSD Cancer Center, University of California at San Diego, La Jolla, California, USA.


A novel time-domain optical method to reconstruct the relative concentration, lifetime, and depth of a fluorescent inclusion is described. We establish an analytical method for the estimations of these parameters for a localized fluorescent object directly from the simple evaluations of continuous wave intensity, exponential decay, and temporal position of the maximum of the fluorescence temporal point-spread function. Since the more complex full inversion process is not involved, this method permits a robust and fast processing in exploring the properties of a fluorescent inclusion. This method is confirmed by in vitro and in vivo experiments.

Copyright 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.

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