Format

Send to

Choose Destination
See comment in PubMed Commons below
FEMS Immunol Med Microbiol. 2010 Jun 1;59(1):71-80. doi: 10.1111/j.1574-695X.2010.00663.x. Epub 2010 Feb 17.

gammadelta T cells promote the maturation of dendritic cells during West Nile virus infection.

Author information

  • 1Department of Microbiology & Immunology, The University of Texas Medical Branch, Galveston, TX 77555-0609, USA.

Abstract

gammadelta T cells are important for the early control of West Nile virus (WNV) dissemination. Here, we investigated the role of gammadelta T cells in the regulation of CD4(+) T-cell response following a WNV challenge. Splenic dendritic cells (DCs) of WNV-infected gammadelta T-cell-deficient (TCRdelta(-/-)) mice displayed lower levels of CD40, CD80, CD86 and major histocompatibility complex (MHC) class II expression and interleukin-12 (IL-12) production than those of wild-type mice. Naïve DCs cocultured with WNV-infected gammadelta T cells showed enhanced levels of costimulatory molecules, MHC class II expression and IL-12 production. Further, coculture of CD4(+) T cells from OT II transgenic mice with DCs of WNV-infected TCRdelta(-/-) mice induced less interferon-gamma (IFN-gamma) and IL-2 production than with those of wild-type controls. Viral antigens were detected in WNV-infected gammadelta T cells.WNV infection or toll-like receptor (TLR) agonist treatment of gammadelta T cells induced the production of IFN-gamma, tumor necrosis factor-alpha and IL-6, which are known to promote DC maturation. Nevertheless, the levels of TLRs 2, 3, 4 and 7 expression of WNV-infected gammadelta T cells were not different from those of noninfected cells. Overall, these data suggest that WNV-induced gammadelta T-cell activation promotes DC maturation and initiates CD4(+) T-cell priming.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk