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J Virol. 2010 Jun;84(11):5520-7. doi: 10.1128/JVI.00125-10. Epub 2010 Mar 24.

Membrane insertion and biogenesis of the Turnip crinkle virus p9 movement protein.

Author information

  • 1Departament de Bioquímica i Biologia Molecular, Universitat de València, E-46 100 Burjassot, Spain. Luis.Martinez-Gil@uv.es

Abstract

Plant viral infection and spread depends on the successful introduction of a virus into a cell of a compatible host, followed by replication and cell-to-cell transport. The movement proteins (MPs) p8 and p9 of Turnip crinkle virus are required for cell-to-cell movement of the virus. We have examined the membrane association of p9 and found that it is an integral membrane protein with a defined topology in the endoplasmic reticulum (ER) membrane. Furthermore, we have used a site-specific photo-cross-linking strategy to study the membrane integration of the protein at the initial stages of its biosynthetic process. This process is cotranslational and proceeds through the signal recognition particle and the translocon complex.

PMID:
20335263
[PubMed - indexed for MEDLINE]
PMCID:
PMC2876587
Free PMC Article
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