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PLoS One. 2010 Mar 19;5(3):e9605. doi: 10.1371/journal.pone.0009605.

The complete genome sequence of Haloferax volcanii DS2, a model archaeon.

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  • 1Department of Biology, Johns Hopkins University, Baltimore, Maryland, United States of America.

Abstract

BACKGROUND:

Haloferax volcanii is an easily culturable moderate halophile that grows on simple defined media, is readily transformable, and has a relatively stable genome. This, in combination with its biochemical and genetic tractability, has made Hfx. volcanii a key model organism, not only for the study of halophilicity, but also for archaeal biology in general.

METHODOLOGY/PRINCIPAL FINDINGS:

We report here the sequencing and analysis of the genome of Hfx. volcanii DS2, the type strain of this species. The genome contains a main 2.848 Mb chromosome, three smaller chromosomes pHV1, 3, 4 (85, 438, 636 kb, respectively) and the pHV2 plasmid (6.4 kb).

CONCLUSIONS/SIGNIFICANCE:

The completed genome sequence, presented here, provides an invaluable tool for further in vivo and in vitro studies of Hfx. volcanii.

PMID:
20333302
[PubMed - indexed for MEDLINE]
PMCID:
PMC2841640
Free PMC Article
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