Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Transplant Proc. 2010 Mar;42(2):444-7. doi: 10.1016/j.transproceed.2010.01.016.

Experimental model of isolated lung perfusion in rats: first Brazilian experience using the IL-2 isolated perfused rat or guinea pig lung system.

Author information

  • 1Department of Cardio-Pulmonology, Heart Institute (InCor), Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil. paulopego@incor.usp.br

Abstract

INTRODUCTION:

Lung transplantation has become the mainstay therapy for patients with end-stage lung disease refractory to medical management. However, the number of patients listed for lung transplantation largely exceeds available donors. The study of lung preservation requires accurate, cost-effective small animal models. We have described a model of ex vivo rat lung perfusion using a commercially available system.

METHODS:

Male Wistar rats weighing 250 g-300 g were anesthetized with intraperitoneal sodium thiopental (50 mg/kg body weight). The surgical technique included heart-lung block extraction, assembly, and preparation for perfusion and data collection. We used an IL-2 Isolated Perfused Rat or Guinea Pig Lung System (Harvard Apparatus, Holliston, Mass, United States; Hugo Sachs Elektronik, Alemanha).

RESULTS:

Preliminary results included hemodynamic and pulmonary mechanics data gathered in the experiments.

CONCLUSION:

The isolated rat lung perfusion system is a reliable method to assess lung preservation.

Copyright (c) 2010 Elsevier Inc. All rights reserved.

PMID:
20304160
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk