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Free Radic Biol Med. 2010 Jul 15;49(2):122-9. doi: 10.1016/j.freeradbiomed.2010.03.009. Epub 2010 Mar 18.

Detection of nitric oxide by electron paramagnetic resonance spectroscopy.

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  • 1Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA. nhogg@mcw.edu


Electron paramagnetic resonance (EPR) spectroscopy has been used in a number of ways to study nitric oxide chemistry and biology. As an intrinsically stable and relatively unreactive diatomic free radical, the challenges of detecting this species by EPR are somewhat different from those of transient radical species. This review gives a basic introduction to EPR spectroscopy and discusses its uses to assess and quantify nitric oxide formation in biological systems.

Copyright 2010 Elsevier Inc. All rights reserved.

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